Zenith Scientific

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Biochemical

Annexin V-FITC Apoptosis Detection Kit

Zenith's Annexin V-FITC Apoptosis Detection kit allows for fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The process needs only a 15-minute incubation procedure.

Annexin V has a high affinity in a Ca2+-dependent manner to negatively charged phospholipid phophatidylserine, which is found on the outer cell membrane early during apoptosis. The Annexin V-FITC kit employs FITC conjugated Annexin V in concert with propidium iodide (PI).

As the cell membrane becomes increasingly permeable during the later stage of apoptosis, propidium iodide can readily move across the cell membrane and bind to DNA. This combination allows the differentiation among 3 populations of cells in two-color flow cytometry:

 

  • Normal cells: Annexin V negative and PI negative
  • Early apoptotic cells: Annexin V positive and PI negative
  • Necrotic cells or late apoptotic cells: Annexin V positive and PI positive.

 

Alternatively, the cell can be examined with a fluorescence microscope equipped with FITC and rhodamine filter sets.

 

Comments:

  • Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

 

Catalogue Number

Pack size

APAPKZ001P0050

50 Assay

APAPKZ001P0100

100 Assay

 

 Surgical Tools

Biopsy forcep

 

Used for myocardial tissue sampling for biopsy.

Unique construction facilitates torque control and provides maximum forcep cutting strength without tip deflection.

Forceps are positioned through an introducer sheath or catheter to obtain biopsy tissue sample under fluoroscopic control.

Supplied sterile in peel-open packages. Intended for one-time use

 

Catalog  No.

Description

Pack size

ENBFSZ101P0001

Biopsy Forceps with spike

1

ENBFWZ101P0001

Biopsy Forceps without spike

1

 

CBD Basket

 

Used for endoscopic removal of CBD stones and foreign bodies.

Catalog  No.

Description

Pack size

ENCBDZ101P0001

CBD Basket

1

 

 

Grasping forceps

 

 

Catalog  No.

Description

Pack size

ENGF2Z101P0001

2 Prong Grasping Forcep

1

ENGF3Z201P0001

3 Prong Grasping Forcep

1

ENGF4Z101P0001

4 Prong Grasping Forcep

1

 

 

Injector needle

 

Used for injection of fluids under direct endoscopic vision. The needle can also be used to manipulate or position a mass during the endoscopic procedure. We offer a complete line up of injection needles to suit aspiration needs in every procedure. Each variety offers excellent puncture capability and outstanding echogenicity. Intended for one-time use only

 

 

Catalog  No.

Description

Pack size

ENINJZ101P0001

Injector Needle 21G

1

ENINJZ102P0001

Injector Needle 23G

1

ENINJZ103P0001

Injector Needle 25G

1

 

Other tools

Catalog  No.

Description

Pack size

ENLIBZ101P0001

Litho Basket

1

ENLIBZ102P0001

Litho Basket with Handle

1

ENPPSZ101P0001

Polypectomy Snear

1

ENRTAZ201P0001

Rat Tooth/alligator

1

ENSGWZ101P0001

Steel Guide Wire

1

ENCCBZ101P0001

Channel Cleaning Brush

1

ENCCBZ102P0001

Channel Cleaning Brush with cover

1

ENCLGZ101P0001

Cytology Brushes

1

ENMLIZ101P0001

Multiband Ligator with cartridge

1

ENMLIZ101P0002

Cartridge (Multiband Ligator)

1

 

 

 

Diagnostic kit

 

Rapid Test

Rapid urase test

Helicobacter pylori is a bacterium found in the gastrointestinal tract of up to 60 percent or two-thirds of the world’s population. Most individuals infected with it will not develop symptoms or be affected by it. However, H. pylori can cause several gastrointestinal disorders, including ulcers, stomach cancer and chronic gastritis (inflammation of the stomach). The bacterium releases enzymes that allow it to invade the lining of the stomach and the first part of the small intestine, called the duodenum. These areas are then more vulnerable to damage from the stomach’s digestive acid and this, in turn, may cause chronic inflammation in the stomach (gastritis) or duodenum (duodenitis), and sometimes ulcer formation. The actual source of H. pylori infection is unknown but is thought to include swimming in, or drinking, fecal-contaminated water, or eating uncooked vegetables. Proper precautions include washing the hands (15-30 seconds with antibacterial soap), drinking clean water from a reliable source and eating only well-cooked foods.

Symptoms of H. pylori infection include the following:

Upper abdominal pain (often described as a burning or gnawing pain)

Nausea and/or vomiting

Decreased appetite

Dark stools (blood passed from the stomach through the intestines produces dark stools)

H. pylori can be diagnosed in a number of ways, including blood tests that look for antibodies made by the body in response to the infection. Other tests include stool studies that detect proteins produced by the bacterium, and a breath test. The breath test involves swallowing a substance that is degraded by the bacteria and can then be detected by sampling a patient’s breath.

Another more invasive method is endoscopy, where a specialized, flexible tube is placed through the mouth, past the esophagus and into the stomach. The tube contains a camera and light that allow the physician to view the esophagus, stomach and duodenum and take biopsies for H. pylori testing.

H. pylori is treated with a combination of at least two antibiotics and a proton-pump inhibitor for 7 to 14 days. The combination of medications has about a 90 percent cure rate when the patient takes all the medication as prescribed.

H. pylori produce large amounts of the enzyme urease. Although the primary function of urease is to allow H. pylori to utilize urea as a nitrogen source, the breakdown of urea also produces high local concentrations of ammonia. When a tissue specimen from a patient is assayed by the HelicoRapt test, the elevated pH level produced by the presence and activity of urease is indicated by a color change in the test paper.

Procedure :

On the front side of the slide record the patient's information. We recommend two slides per patient for confirmation of the result.
The total procedure is delineated on the back side of the slide.
Open the test slide.
Transfer the biopsy sample onto the test paper using a sterile needle.

Re-seal the slide.

Press the top cover over the test paper to squeeze the fluid out of the biopsy sample.

Monitor the test slide over the course of an hour. Observe any color change through the window on the back of the test slide.

 

 

Catalog No

Description

Pack size

RAHPYZ001P0050

HelicoRapt (rapid detection kit for H. pylori)

50

RAHPYZ001P0100

HelicoRapt (rapid detection kit for H. pylori)

100

 

 Real-time PCR based test

Traditionally, scientists and clinicians have used assays that involve growing cultures to screen samples for the presence of pathogenic microorganisms. Today, culture- based methods for pathogen detection are rapidly being replaced by faster and more specific Real Time PCR assays that discriminate between microorganisms based on a signal from specific nucleic acid sequences.

We have developed high quality Real Time PCR assays for various human pathogens and molecular diseases. The kits contain both extraction reagents and real time PCR reagents with positive controls. They are designed with the broadest possible detection profile from detailed sequence alignments of representative members of the strain/subtype.

Components of core kit are:

  1. Specific primers and probe in 20X formulation.
  2. 2X PCR mastermix.
  3. Reagents for cDNA conversion (if applicable)
  4. Linearized plasmid standard (10M copies per mL) for 10 standard curves

 Components of total kit are:

  1. Core kit.
  2. Nucleic acid extraction reagent.

 

Catalog No

Description

Pack size

PHBKVZ002P0100

BKV detection core kit (qualitative)

100 reactions

PHBKVZ003P0100

BKVdetection total kit (qualitative)

100 reactions

PHBKVZ004P0100

BKV detection core kit (quantitative)

100 reactions

PHBKVZ005P0100

BKV detection total kit (quantitative)

100 reactions

PHHBVZ002P0100

HBV detection core kit (qualitative)

100 reactions

PHHBVZ003P0100

HBV detection total kit (qualitative)

100 reactions

PHHBVZ004P0100

HBV detection core kit (quantitative)

100 reactions

PHHBVZ005P0100

HBV detection total kit (quantitative)

100 reactions

PHHCVZ002P0100

HCV detection core kit (qualitative)

100 reactions

PHHCVZ003P0100

HCV detection total kit (qualitative)

100 reactions

PHHCVZ004P0100

HCV detection core kit (quantitative)

100 reactions

PHHCVZ005P0100

HCV detection total kit (quantitative)

100 reactions

PHHIVZ002P0100

HIV1 detection core kit (qualitative)

100 reactions

PHHIVZ003P0100

HIV1 detection total kit (qualitative)

100 reactions

PHHIVZ004P0100

HIV1 detection core kit (quantitative)

100 reactions

PHHIVZ005P0100

HIV1 detection total kit (quantitative)

100 reactions

PHHIVZ006P0100

HIV2 detection core kit (qualitative)

100 reactions

PHHIVZ007P0100

HIV2 detection total kit (qualitative)

100 reactions

PHHIVZ008P0100

HIV2 detection core kit (quantitative)

100 reactions

PHHIVZ009P0100

HIV2 detection total kit (quantitative)

100 reactions

PHHLAZ002P0100

HLA-B27 detection core kit

100 reactions

PHHLAZ003P0100

HLA-B27 detection total kit

100 reactions

PHHPVZ002P0100

HPV16 detection core kit (qualitative)

100 reactions

PHHPVZ003P0100

HPV16 detection total kit (qualitative)

100 reactions

PHHPVZ004P0100

HPV16 detection core kit (quantitative)

100 reactions

PHHPVZ005P0100

HPV16 detection total kit (quantitative)

100 reactions

PHHTLZ002P0100

HTLV1 detection core kit (qualitative)

100 reactions

PHHTLZ003P0100

HTLV1detection total kit (qualitative)

100 reactions

PHHTLZ004P0100

HTLV1 detection core kit (quantitative)

100 reactions

PHHTLZ005P0100

HTLV1 detection total kit (quantitative)

100 reactions

PHHTLZ006P0100

HTLV2 detection core kit (qualitative)

100 reactions

PHHTLZ007P0100

HTLV2 detection total kit (qualitative)

100 reactions

PHHTLZ008P0100

HTLV2 detection core kit (quantitative)

100 reactions

PHHTLZ009P0100

HTLV2 detection total kit (quantitative)

100 reactions

PHMTbZ002P0100

MTb detection core kit (qualitative)

100 reactions

PHMTbZ003P0100

MTb detection total kit (qualitative)

100 reactions

PHMTbZ004P0100

MTb detection core kit (quantitative)

100 reactions

PHMTbZ005P0100

MTb detection total kit (quantitative)

100 reactions

PHPMLZ002P0100

PML-RARA detection core kit

100 reactions

PHPMLZ003P0100

PML-RARA detection total kit

100 reactions

PHAMLZ002P0100

AML-ETO detection core kit

100 reactions

PHAMLZ003P0100

AML-ETO detection total kit

100 reactions

PHBCRZ002P0100

BCR-Abl detection core kit

100 reactions

PHBCRZ003P0100

BCR-Abl detection total kit

100 reactions